ABSTRACT
Se conoce como fibromatosis desmoide a la proliferación fibroblástica clonal que surge de partes blandas y se caracteriza por un crecimiento infiltrativo, localmente invasivo y con tendencia a la recurrencia local, pero con baja capacidad de metastatizar. Es un patología infrecuente en la edad padiatrica, sobre todo en la localización de cabeza y cuello. su tratamiento esta en discusión entre la cirugía radical, la conducta expectante y la radio o quimioterapia. Se presenta el caso clínico de un varón de 4 años de edad con diagnostico de fibromatosis desmoide laterocervical de cuello, adyacente a paquete vascular yugulocarotideo, en el cual se practico con éxito la resección total.
Desmoid fibromatosis is a fibroblastic monoclonal proliferation of soft tissues, and is characterized by an infiltrative growth, locally invasive and with tendency to local recurrence, but with a low metastatic capacity . It is an uncommon condition in pediatric patients, especially in head and neck locations. The treatment is under discussion between radical surgery, expectant management and radio or chemotherapy. We report the clinical case of a 4 years old male with a diagnosis of laterocervical desmoid fibromatosis of the neck, adjacent to a vascular jugulocarotide package, in which the total resection was successfully performed.
Subject(s)
Humans , Male , Child, Preschool , Radiotherapy , Fibromatosis, Aggressive/surgery , Fibromatosis, Aggressive/therapy , Drug Therapy , Margins of Excision , Head and Neck Neoplasms/pathologyABSTRACT
Avaliaram-se a proliferação de linfócitos e a apoptose de células CD5+ de bovinos naturalmente infectados pelo vírus da leucose enzoótica bovina. Para tal, 100 vacas da raça Holandesa, em lactação, foram triadas quanto ao sorodiagnóstico para a leucose enzoótica bovina e o perfil hematológico, e 15 foram escolhidos e distribuídos uniformemente entre os três grupos, a saber: animais negativos, animais positivos alinfocitóticos e animais positivos e que manifestaram linfocitose persistente (LP). Para a avaliação da proliferação de linfócitos, procedeu-se ao isolamento das células mononucleares por gradiente de centrifugação, em que 2x10(6) linfócitos por mL foram plaqueados por poço e analisados por citometria de fluxo utilizando-se o fluorocromo CFSE-DA. A apoptose do sangue periférico deu-se utilizando a anexina V-FITC, e para a identificação das células CD5+, utilizaram-se anticorpos monoclonais. Ocorreu menor proliferação de linfócitos nos animais infectados e que manifestavam LP, e menor apoptose de células CD5+ do sangue periférico. Pode-se sugerir que o desenvolvimento da LP, resultante do aumento de linfócitos B, deve-se à redução do processo apoptótico das células CD5+, principal população infectada, e que a maior proliferação linfocitária pode se restringir apenas ao estádio inicial do desenvolvimento da LP.
The purpose of the present trail was to evaluate the lymphocyte proliferation and the apoptosis rates of CD5+ cells in dairy cows infected with bovine leukemia virus (BLV) with distinct lymphocyte profiles in infected animals known as alymphocytotic (AL) and persistent lymphocytosis (PL). A total of 100 Holstein cows were sera tested for bovine leukemia virus through agar gel immunodiffusion (AGID) and enzyme-linked immunosorbent-assay (ELISA). From these animals, 15 cows were selected and divided uniformly in 3 groups (negative, AL, LP). The lymphocyte proliferation was performed using flow cytometric measurement of CFSE-DA dye, where 2x10(6)/mL lymphocytes were plated per well. The apoptosis of CD5+ cells from peripheral blood was performed using the annexin V-FITC to measure the apoptosis rates and the identification of CD5+ was accessed using monoclonal antibodies. Animals from the LP group showed lower lymphocyte proliferation and also lower apoptosis rates of CD5+ cells compared with negative and AL animals. The development of PL which resulted from an increase in B cell count, is due to the decrease in the apoptosis rates of CD5+ cells, and the higher lymphocyte proliferation appears to be limited only in the initial stages of development of LP.